| Congresso Brasileiro de Microbiologia 2023 | Resumo: 843-1 | ||||
Resumo:The resistance of many bacteria to antibiotics has caused a great stir in the last decades. This resistance is expressed through different mechanisms that help bacteria combat various drugs, including enzymatic degradation, efflux pumps, or target disruption. Bacteria are found in any environment, but you can see more human exposure with the water and food consumed. A recent study detected bacteria resistant to various antibiotics in food exported from the Dominican Republic. This opens the possibility that they may also affect the local population. For this reason, it is essential to know the different pathogenic bacteria present in the foods consumed in the daily life of Dominicans, as well as the various resistances to antibiotics that they can present to address this problem better. In this study, samples of three vegetables consumed raw were collected: cabbage lettuce (Lactuca sativa), tomato (Solanum lycopersicum), and cabbage (Brassica oleracea var. capitata) from three establishments in the province of Santo Domingo to chromogenically identify and using the MALDI-TOF technique, species of bacteria, in addition to estimating the bacterial load. A total of 24 strains of bacteria were obtained, of which the most frequent genera were Bacillus spp, followed by Klebsiella ssp , Acinetobacter spp. , and Enterobacter ssp . From the 24 strains, three species of bacteria were chosen to carry out DNA extraction and sequencing. In the resistance profiles of the sequenced bacteria, a significant similarity was observed, as they possessed these multiple resistance mechanisms with subsystems corresponding to beta-lactamases and efflux pumps, and, on the other hand, the resistance genes found were OXY- 6-2, poxtA, fexB, and AbaQ.It is essential to highlight that there is a disparity in the bacterial load of the food in the different establishments where a higher concentration was observed in the public market, so it can be inferred that the environment where they were collected influenced these results. Palavras-chave: resistance, vegetables for raw consumption , Dominican Republic, Santo Domingo Agência de fomento: Fondo Nacional de Innovación y Desarrollo Científico y Tecnológico-FONDOCYT | |||||